New Product Launch | TruArray™ High-Throughput DNA Microarray is Now Available! Embrace the Emergence!

TruArray™ Series

Product Categories and Technical Specifications

High-throughput DNA synthesis products are typically delivered in two mainstream forms: Oligonucleotide Microarray or Oligo Array, where the nucleic acid products remain in situ on the chip surface, and Oligo Pool, where the oligonucleotide arrays are cleaved from the chip surface for use.

The "impossible quadrilateral" of DNA synthesis — high (high throughput, high unit yield), fast (fast production, short delivery cycle), good (high accuracy, good uniformity), economical (low customization threshold, low mass production cost) — remains a challenge. Different application scenarios also have varying preferences for technical specifications and delivery forms.

Based on TFT semiconductor and electrochemical technologies, LinkZill can deliver high-quality products in two categories: 「 TruArray™ 」theseries oligonucleotide microarrays and 「 TruPool™ 」 series oligo pool. This achieves an unprecedented balance among various technical parameters.

Today, LinkZill has launched its first standardized product based on the TFT-DNA synthesis technology—TruArray™ Std 4K High-Throughput Oligonucleotide Microarray Chip.

TruArray™ Series

Standard Product Categories

[1] For availability and specific requirements, please consult our customer service manager at info@linkzill.com.

[2] RNA/XNA microarrays or DNA microarrays with 5' end coupling on the chip surface are expected to be launched by the end of 2025.

[3] Microarray chips with array sizes up to 65K are expected to be available by Q1 2025.

[4] Microarray chips with array sizes up to 131K are expected to be available by Q2 2025.

TruArray™ Std 4K microarray

Technical Specifications

Currently, the standard version TruArray™ Std 4K microarray, with oligonucleotide lengths up to 150 nt, is now open for customization.

TruArray™ Series

Technical Progress

For oligonucleotide microarray, coverage, uniformity between spots, and synthesis throughput are critical technical indicators. The limitations in synthesis length and probe loading capacity of existing commercial services have also become bottlenecks for many emerging applications. 「 TruArray™ 」series has achieved internationally leading technical indicators.

• Coverage Reaches Globally Leading Quality Standards

The 4K throughput microarray coverage stably exceeds 99.9%, effectively resolving common issues such as bad rows, bad columns, and bad areas seen in other electrochemical technology routes, reaching globally leading quality standards.

• Uniformity at Globally Leading Levels

On a 4K chip, 4096 random 100 nt oligonucleotide arrays were synthesized for FISH hybridization and uniformity evaluation. 90% of the spots' raw fluorescence values fall within a 1.3-fold range, and 99% of the spots' raw fluorescence values fall within a 1.5-fold range (Q95:Q5 < 1.3X, Q99.5:Q0.5 < 1.5X, fluorescence values after log₂ transformation: Q95:Q5 < 1.03, Q99.5:Q0.5 < 1.05), surpassing publicly available data from comparable global services (world-leading inkjet-printed Oligo Pool product data: Q95:Q5 < 1.9X, Q99.5:Q0.5 < 3.5X, though it should be noted that evaluation methods differ).

/ Figure: TruArray™ Std 4K 100 nt Oligonucleotide Microarray Uniformity Test /

• Synthesis Length Reaches Globally Leading Levels

Stable synthesis of 150 nt DNA has been achieved on 4K throughput chips, with tested synthesis lengths exceeding 300 nt, significantly surpassing the typical 25–60 nt range of globally commercialized oligonucleotide microarray customization services.

• Synthesis Loading Capacity is Flexible and Customizable

Depending on the chip design, the probe loading capacity per spot in 4K throughput microarrays can reach 0.1–3 fmol, with both maximum loading capacity and flexibility surpassing existing high-throughput DNA synthesis technologies.

• Synthesis Throughput

The LinkZill TruArray™ Std 4K is now open for customization, with each chip capable of synthesizing up to 4,096 oligonucleotides; 65K chips have successfully completed the synthesis of over 100 nt DNA microarrays (coverage > 99.98%, Q95:Q5 < 1.6X, Q99.5:Q0.5 < 2.1X), fully entering the synthesis process optimization phase, and are expected to be open for customization by Q1 2025; 131K chips have completed tape-out and post-processing, and testing has commenced.

/ Left Figure: 65K TFT-DNA chip synthesis of LinkZill and XiaoZhi patterns, Right Figure: The first batch of 131K synthesis chips completed tape-out and post-processing, testing initiated /

TruArray™ Series

Application Scenarios

The technical capabilities of oligonucleotide microarray chips include:

1. In situ hybridization, macromolecular affinity and detection

2. Solid-phase capture

3. Providing spatial coordinates, single-cell or single-molecule labelling, and other nucleic acid encoding information

4. Solid-phase in situ enzymatic reactions

5. Genotyping

   
   

6. On-chip in situ sensing

Based on these technical capabilities, the TruArray™ series can achieve the following application scenarios, with delivered angel customers including:

1. High-throughput pathogen and resistance rapid screening

   Delivered to 1 leading IVD enterprise industrial customer;

   
   

2. High-throughput antibody and aptamer discovery

   In situ nanobody library construction, delivered to 1 national laboratory academic customer;

   High-throughput aptamer discovery, delivered to 1 academic customer from the Chinese Academy of Sciences system;

   
   

3. In situ gene splicing and protein expression

   Delivered to 1 synthetic biology CRO enterprise industrial customer and 1 provincial laboratory academic customer;

   
   

4. In situ spatial omics

   Delivered to 1 national laboratory academic customer;

   
   

5. Non-mass spectrometry proteomics technology development

   Delivered to 1 academic customer from the Chinese Academy of Sciences system;

   
   

6. Genomic variation and methylation typing

   Methylation typing technology development, delivered to 1 national laboratory academic customer;

   
   

7. In situ DNA information storage and reading

   Delivered to 1 double-first-class university academic customer;

   
   

8. Sequencing technology validation

   Nanopore sequencing technology development, delivered to 1 double-first-class university academic customer;

   
   

9. Gene expression profiling

   
   

10. Other medical diagnostics

TruPool™ Series

Technical Progress

Alongside the release of the TruArray™ series, Announcing another major new product from LinkZill—「 TruPool™ 」 series oligo pool, expected to be open for customization by Q1 2025.

For oligo pool, synthesis length, accuracy, yield per oligonucleotide, and uniformity are critical technical indicators. As TFT-DNA synthesis technology continues to optimize and iterate, the quality of LinkZill 「 TruPool™ 」 series oligonucleotide pools is steadily improving, covering various delivery forms and technical specifications.

• Synthesis Throughput and Length

4K throughput, 120 nt oligonucleotide pool products have completed multiple rounds of NGS sequencing validation, and 65K throughput, 200 nt oligo pool products are about to undergo sequencing validation.

• Coverage

Stably exceeds 99%.

• Accuracy Continues to Improve

With continuous optimization of equipment, chips, reagents, synthesis processes, and post-processing technologies, the single-base error rate has steadily decreased, currently reaching around 0.5%, close to column synthesis levels (approximately 0.1–0.3%), with further optimization potential; existing data already surpasses literature-reported commercialized electrochemical (approximately 1.6%) [1] and photochemical technology routes (1% to over 10%) [2], and measured results for selected inkjet printing technology routes (note that sample parameters and processing methods may differ).

/Figure: NGS sequencing validation shows that the synthesis error rate of TruPool™ 4K 100 nt   oligo pool steadily decreases with chip and synthesis process iterations /

• Uniformity Continues to Improve

After 10, 20, and 30 rounds of PCR amplification, the Q95:Q5 of reads per synthesized sequence in the 4K throughput oligo pool is less than 11.0X, 21.5X, 28.0X respectively (reads after log₂ transformation: 1.5X, 1.6X, 1.8X, Shapiro normality: 0.59, 0.50, 0.52); Superior technical specifications compared to the world's leading inkjet printing-based synthetic product measurements reported in the literature (10 PCR amplification cycles, Q95:Q5: TruPool™ 11.0X < benchmark product 17.0X, Shapiro normality TruPool™ 0.59 > benchmark product 0.50; note that sample parameters and processing methods may differ) [3].

/Figure: NGS sequencing validation shows that TruPool™ 4K 100 nt oligo pool maintains good uniformity after multiple rounds of PCR /

References:

[1] Gimpel, A. L., Stark, W. J., Heckel, R., & Grass, R. N. (2023). A digital twin for DNA data storage based on comprehensive quantification of errors and biases. Nature Communications, 14(1), 6026.

[2] Antkowiak, P. L., Lietard, J., Darestani, M. Z., Somoza, M. M., Stark, W. J., Heckel, R., & Grass, R. N. (2020). Low cost DNA data storage using photolithographic synthesis and advanced information reconstruction and error correction. Nature communications, 11(1), 5345.

[3] Gao, Y., Chen, X., Qiao, H., Ke, Y., & Qi, H. (2020). Low-bias manipulation of DNA oligo pool for robust data storage. ACS synthetic biology, 9(12), 3344-3352.

Interdisciplinary Team and Industry Chain Advantages

Drive 「LinkZill Speed」

Thanks to LinkZill's rich accumulation of technology modules and interdisciplinary R&D capabilities, as well as the synergy between TFT-DNA technology selection and China's strong TFT semiconductor industry, since the launch of LinkZill's life science semiconductor business, the first standardized product of TFT-DNA technology was officially introduced after just over a year of R&D iteration, continuously demonstrating 「LinkZill Speed」.

In the past year:

• 11 times of TFT-DNA synthesis chip tape-out were implemented.

• 67 types of chip backplane verification were completed.

• 5,420 TFT-DNA synthesis chips were produced and processed.

• Over 10 million oligonucleotides were synthesized.

• 3 types of semiconductor materials were involved in tape-out.

• 206 types of chip film layer and graphic design were used.

• Over 4,000 chip synthesis experiments were completed.

• Over 640 million bases were synthesized.

At the same time, we welcomed the sixth-generation Alpha test model of the TFT-DNA synthesiser and the first-generation Beta test model, bringing the number of online synthesizers to double digits.

TruArray™ Series

Consultation and Customization Portal

• Product Information Download   

Click the link https://m.linkzill.com/cn/col.jsp?id=156 to access the product manual for TruArray™.   

• Ordering Information   

If you need to order our products or learn more details, please contact us via email at info@linkzill.com.   

• Ordering Instructions   

LinkZill's TFT-DNA synthesis technology is still in a phase of rapid R&D. Chip design, synthesis processes, and product quality are continuously iterating, and the product matrix is rapidly being validated and expanded. As a result, our current capacity to serve customized customer demands in terms of manpower and equipment availability remains limited. We will evaluate your requirements and further confirm the collaboration approach with you.

We welcome more angel customers to help us further improve the technical indicators of the TruArray™ series, expand its application scenarios, and open up a wider space of imagination. Join us in linking technology and leading the transformation of life science productivity.